Gateway vectors for plant transformation
نویسندگان
چکیده
منابع مشابه
Update on Recombinational Cloning with Plant Gateway Vectors Recombinational Cloning with Plant Gateway Vectors
The study of biological systems relies to a large extent on DNA cloning technologies enabling the analysis of recombinant genes through transgenic research. In this context, the advent of recombinational cloning methods was a significant progress because DNA fragments can now be assembled regardless of their sequence. In particular, the Gateway system was designed to join fragments in a predefi...
متن کاملGateway-compatible vectors for plant functional genomics and proteomics.
Gateway cloning technology facilitates high-throughput cloning of target sequences by making use of the bacteriophage lambda site-specific recombination system. Target sequences are first captured in a commercially available "entry vector" and are then recombined into various "destination vectors" for expression in different experimental organisms. Gateway technology has been embraced by a numb...
متن کاملGateway Vectors for Plant Genetic Engineering: Overview of Plant Vectors, Application for Bimolecular Fluorescence Complementation (BiFC) and Multigene Construction
Yuji Tanaka1, Tetsuya Kimura2, Kazumi Hikino3, Shino Goto3,4, Mikio Nishimura3,4, Shoji Mano3,4 and Tsuyoshi Nakagawa1 1Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, 2Department of Sustainable Resource Science, Graduate School of Bioresources, Mie University, 3Department of Cell Biology, National Institute for Basic Biology, 4Dep...
متن کاملNovel plant transformation vectors containing the superpromoter.
We developed novel plasmids and T-DNA binary vectors that incorporate a modified and more useful form of the superpromoter. The superpromoter consists of a trimer of the octopine synthase transcriptional activating element affixed to the mannopine synthase2' (mas2') transcriptional activating element plus minimal promoter. We tested a superpromoter-beta-glucuronidaseA fusion gene in stably tran...
متن کاملConstruction of Plant Expression Vectors Harboring WRI1 and LPAAT Genes and Its Transformation in Tobacco Plants
In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic tran...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2009
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.26.275